Conda environments

Conda is an open source package and environment management system that simplifies the installation and the configuration of complex software packages in a platform agnostic manner.

Nextflow has built-in support for Conda that allows the configuration of workflow dependencies using Conda recipes and environment files.

This allows Nextflow applications to use popular tool collections such as Bioconda whilst taking advantage of the configuration flexibility provided by Nextflow.


This feature requires Nextflow version 0.30.x or higher and the Conda or Miniconda package manager installed on your system.

How it works

Nextflow automatically creates and activates the Conda environment(s) given the dependencies specified by each process.

Dependencies are specified by using the conda directive, providing either the names of the required Conda packages, the path of a Conda environment yaml file or the path of an existing Conda environment directory.


Conda environments are stored on the file system. By default Nextflow instructs Conda to save the required environments in the pipeline work directory. Therefore the same environment can be created/saved multiple times across multiple executions when using a different work directory.

You can specify the directory where the Conda environments are stored using the conda.cacheDir configuration property (see the configuration page for details). When using a computing cluster, make sure to use a shared file system path accessible from all computing nodes.


The Conda environment feature is not supported by executors which use a remote object storage as a work directory eg. AWS Batch.

Use Mamba to resolve packages

It is also possible to use mamba for speeding up creation of conda environments. For more information on how to enable this feature please refer Conda.


The use of mamba to create conda environments is an experimental feature and the functionality may change in future releases.

Use Conda package names

Conda package names can specified using the conda directive. Multiple package names can be specified by separating them with a blank space. For example:

process foo {
  conda 'bwa samtools multiqc'

  your_command --here

Using the above definition a Conda environment that includes BWA, Samtools and MultiQC tools is created and activated when the process is executed.

The usual Conda package syntax and naming conventions can be used. The version of a package can be specified after the package name as shown here bwa=0.7.15.

The name of the channel where a package is located can be specified prefixing the package with the channel name as shown here bioconda::bwa=0.7.15.

Use Conda environment files

Conda environments can also be defined using one or more Conda environment files. This is a file that lists the required packages and channels structured using the YAML format. For example:

name: my-env
  - conda-forge
  - bioconda
  - defaults
  - star=2.5.4a
  - bwa=0.7.15

Read the Conda documentation for more details about how to create environment files.

The path of an environment file can be specified using the conda directive:

process foo {
  conda '/some/path/my-env.yaml'

  your_command --here


The environment file name must end with a .yml or .yaml suffix otherwise it won’t be properly recognised.

Alternatively it is also possible to provide the dependencies using a plain text file, just listing each package name as a separate line. For example:



Like before the extension matter, make sure such file ends with the .txt extension.

Use existing Conda environments

If you already have a local Conda environment, you can use it in your workflow specifying the installation directory of such environment by using the conda directive:

process foo {
  conda '/path/to/an/existing/env/directory'

  your_command --here

Best practices

When a conda directive is used in any process definition within the workflow script, Conda tool is required for the workflow execution.

Specifying the Conda environments in a separate configuration profile is therefore recommended to allow the execution via a command line option and to enhance the workflow portability. For example:

profiles {
  conda {
    process.conda = 'samtools'

  docker {
    process.container = 'biocontainers/samtools'
    docker.enabled = true

The above configuration snippet allows the execution either with Conda or Docker specifying -profile conda or -profile docker when running the workflow script.

Advanced settings

Conda advanced configuration settings are described in the Conda section on the Nextflow configuration page.